Affinity resins have been widely used for the purification of enzymes that bind nucleotides and related molecules. Resins in which ATP is linked via the gamma-phosphate have been valuable in identifying proteins in the purine-binding proteome, which includes kinases, heat shock proteins and other ATP-binding proteins.
ATP Agarose resin comprises ATP attached to agarose beads via its gamma-phosphate. Two forms of the resin are available with low and high ligand substitution. A long hydrophilic spacer (14-atom) is used to minimise unwanted hydrophobic interactions and to facilitate unhindered interactions with biomolecules.
Our ATP Agarose is unique due to the orientation of the nucleotide on the resin. The nucleotide is covalently attached via the terminal phosphate group and this protects the ATP from phosphatase attack and makes the resin extremely stable. The resin can therefore be reused multiple times. More benefits include:
- Quick and easy-to-use
- Extremely stable
- Flexible, they can be used in column chromatography and batch processing
- Consistent from batch-to-batch
We have over 35 publications citing the successful use of our ATP and GTP Agarose. View the full list of references here.
The Control Resin is just the agarose with no ATP bound.
- The THERMOSENSITIVE MALE STERILE 1 Interacts with the BiPs via DnaJ Domain and Stimulates Their ATPase Enzyme Activities in Arabidopsis - PLoS One (2015)
- Synthesis and Evaluation of a Novel Deguelin Derivative, L80, which Disrupts ATP Binding to the C-Terminal Domain of Heat Shock Protein 90 - Molecular Pharmacology (2015)
- A structure-specific nucleic acid-binding domainconserved among DNA repair proteins - PNAS (2014)
- Moyamoya disease-associated protein mysterin/RNF213 is a novel AAA+ ATPase, which dynamically changes its oligomeric state- Scientific Reports (2014)
- Thr90 phosphorylation of Hsp90? by protein kinase A regulates its chaperone machinery - Biochem. J. (2012)
- Functional Characterization of Naturally Occurring Transglutaminase 2 Mutants Implicated in Early-Onset Type 2 Diabetes- Journal of Molecular Endocrinology (2012)
- A Conserved Non-canonical Motif in the Pseudoactive Site of the ROP5 Pseudokinase Domain Mediates Its Effect on Toxoplasma Virulence - JBC (2011)
- Evolution of functional nucleic acids in the presence of nonheritable backbone heterogeneity- PNAS (2011)
- Compositions and Methods for Inhibiting Cancer Metastasis - US Patent Application 20100111943
- Regulation of Calcium/Calmodulin-dependent Kinase IV by O-GlcNAc Modification - J Biol Chem (2009)
- (?)-Epigallocatechin-3-gallate Is a Novel Hsp90 Inhibitor- Biochemistry (2009)
- Role of Acetylation and Extracellular Location of Heat Shock Protein 90? in Tumor Cell Invasion- Cancer Research (2008)
- HDAC6 inhibition enhances 17-AAG–mediated abrogation of hsp90 chaperone function in human leukemia cells - Blood (2008)
- Cryopyrin/NALP3 binds ATP/dATP, is an ATPase, and requires ATP binding to mediate inflammatory signaling - PNAS (2007)
- Phosphorylation and 14-3-3 binding of Arabidopsis trehalose-phosphate synthase 5 in response to 2-deoxyglucose- The Plant Journal (2006)
- The Kinase Homology Domain of Receptor Guanylyl Cyclase C:? ATP Binding and Identification of an Adenine Nucleotide Sensitive Site - Biochemistry (2006)
- Deguelin analog SH-1242 inhibits Hsp90 activity and exerts potent anticancer efficacy with limited neurotoxicity - Cancer Res. (2015)
- ARD1-mediated Hsp70 acetylation balances stress-induced protein refolding and degradation - Nature Commun. (2016)