Affinity resins have been widely used for the purification of enzymes and other proteins that bind nucleotides and related molecules. GTP-agarose resin comprises GTP attached to agarose beads via its ?-phosphate. A long hydrophilic spacer (14-atom) is used to minimise unwanted hydrophobic interactions and to facilitate unhindered interactions with biomolecules. The ligand is coupled through the ?-phosphate group which means that the resin is resistant to phosphatases found in many crude tissue extracts.
The Control Resin is just the agarose with no GTP bound.
- Comprehensive experimental fitness landscape and evolutionary network for small RNA - PNAS (2013)
- Functional Characterization of Naturally Occurring Transglutaminase 2 Mutants Implicated in Early-Onset Type 2 Diabetes - Journal of Molecular Endocrinology (2012)
- GTP-dependent packing of a three-helix bundle is required for atlastin-mediated fusion - PNAS (2011)
- Re-examination of the dimerization state of leucine-rich repeat kinase 2:
predominance of the monomeric form - Biochemical Journal (2011)
- Recruitment of the Earliest Component of the Bacterial Flagellum to the Old Cell Division Pole by a Membrane-Associated Signal Recognition Particle Family GTP-Binding Protein - J Mol Biol (2009)
- High-level production and characterization of a G-protein coupled receptor signaling complex - FEBS Journal (2009)
- G Protein-coupled Receptor-promoted Trafficking of G?1?2 Leads to AKT Activation at Endosomes via a Mechanism Mediated by G?1?2-Rab11a Interaction - Molecular Biology of the Cell (2008)
- Altered Axonal Mitochondrial Transport in the Pathogenesis of Charcot-Marie-Tooth Disease from Mitofusin 2 Mutations - Journal of Neuroscience (2007)
- A Novel Lysine-rich Domain and GTP Binding Motifs Regulate the Nucleolar Retention of Human Guanine Nucleotide Binding Protein, GNL3L - J Mol Biol (2006)