Protein A Agarose (851-0024)
Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species. Immobilization of protein A creates an affinity resin that can be used to isolate IgG fractions from crude serum, ascites fluid or hybridoma supernatants. Aliquots of Protein A resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. Protein A resin is prepared by coupling highly purified protein A to agarose beads.
For more information, please read our Antibody Purification Guide.
- High-Affinity IgG Antibodies Develop Naturally in Ig-Knockout Rats Carrying Germline Human IgH/Igк/Igλ Loci Bearing the Rat CH Region - The Journal of Immunology (2013)
- Sialic Acid within the Glycosylphosphatidylinositol Anchor Targets the Cellular Prion Protein to Synapses - The Journal of Biological Chemistry (2016)