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Lightning-Link® R-Phycoerythrin (RPE)

 

Lightning-Link® R-Phycoerythrin (R-PE)

Lightning-Link® antibody labeling kits enable the direct labeling of antibodies, proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits.

Our R-PE antibody labeling kit enables the direct conjugation of R-PE to any biomolecule with an available amine group. The researcher simply pipettes the antibody or other biomolecule into the vial of Lightning-Link® R-PE and incubates for 3 hours (for more details watch the video below). 

Benefits of Lightning-Link® include:

Features Benefits
Quick and easy to use Save time, no special knowledge required
No separation steps 100% recovery - no antibody/protein loss
Can be used in a wide range of applications Flexible
Freeze dried Ships at ambient temperature, long shelf-life
Fully scalable (10ug to 1g or more) Easy transfer from R&D to manufacturing
Stringently QC tested Consistent high quality, excellent batch-to-batch reproducibility
Large number of labels available Experimental flexibility
Reliable: nearly 300 references Successfully used in many fields of research

Do you want to know if your conjugation was a success?

Try our new and unique Conjugate Check&Go! Kit.

Button-CheckandGo-min

More about R-Phycoerythrin (RPE)

R-Phycoerythrin (R-PE) is a fluorescent protein from the phycobiliprotein family, present in red algae and cryptophytes. It has three maximal absorbance values of 498, 544 and 566nm (the optimal will depend on the application), and it has a strong emission peak at 580nm. RPE is closely related to B-Phycoerythrin (B-PE) and these are the most intense fluorescent phycobiliproteins providing an orange fluorescence.

RPE conjugates are commonly used for immunofluorescence and flow cytometry and are ideal for multiple labeling with other fluorochromes.

Click here to view other fluorescent proteins and dyes available in the Lightning-Link® range.

RPE free sample banner 2

 Please do not hesitate to Contact us to find out more!

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Custom labeling services 

Are you looking to increase assay sensitivity, decrease background or increase stability? Do you require bulk quantities to suit your manufacturing or research needs?

At Innova we can help with all your bioconjugation requirements, so if you can't find what you’re looking for from our range of products, or need something a bit different or at a larger scale, our range of custom services may be of interest to you. These include:

Flow cytometry data comparing direct and indirect labeling

Indirect labeling with CD45RC supernatant

           Indirect labeling with              CD45RC purified Ab

Direct labeling with Lightning-Link CD45RC-PE Purified Ab

 

 

 

 

 

 

 

Flow cytometry data


rpe flow profile

Mouse anti-human CD8 was conjugated with R-Phycoerythrin using an Innova Biosciences Lightning-Link® kit.   The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line – negative control; red line – positive staining).

Which label should I use?

The choice of label for your antibody primarily depends upon the application:

Immunoassay Label
Western Blotting
  • Enzymes (usually HRP or Alkaline Phosphatase)
ELISA
  • Enzymes
  • Biotin/Streptavidin
Immunofluorescence
  • Fluorescent dyes
Immunohistochemistry
  • Enzymes
  • Biotin/Streptavidin
Flow Cytometry
  • Fluorescent proteins
  • Fluorescent dyes
  • Tandem dyes

Please see the table here for absorption/emission wavelengths of Lightning-Link® fluorescent dyes, proteins and tandems.

What can I label using Lightning-Link?

Lightning-Link® technology works by targeting free amine groups on your target. It can be used to label antibodies, peptides, proteins and any other molecules with free amine groups.

For advice on labeling molecules other than antibodies please contact our technical support team.

How pure does my antibody need to be?

Your antibody must be purified because other molecules with free amine groups will interfere with the reaction resulting in a poor quality conjugate.

A suitable method of purification should be selected:

  • Affinity purification - this is the preferred method as it will always result in a purified sample containing only the antibody of interest.
  • Protein A or G purification of serum - this will result in a purified sample of all IgG’s contained in the serum. You will have a purified IgG fraction but only a small percentage of this fraction will be the IgG of interest. This IgG fraction will label with our kits, although adjustments in conjugate dilutions will be required.
  • Protein A or G purification of tissue culture supernatant or ascites fluid - this method will generate purified antibodies equivalent to affinity purification.

Other purification methods, such as ion exchange chromatography and ammonium sulphate precipitation, are low quality purification methods and should be avoided. Filtration using a 0.2/0.22 µm filter is a method of sterilization not purification, and is therefore not appropriate.

Is my antibody in a suitable buffer?

For recommended buffer conditions please see the below table:

ll - dyes - components

 1 Please note that individually the concentrations shown should not affect the reaction. However in combination with additional compounds that are not recommended above a certain concentration, the reaction may be affected.

2 If intending to use this kit for immunohistochemistry, it is recommended that there be no gelatin or BSA present.

What can I do if my antibody formulation does not fit the requirements?

If your antibody requires purification or the buffer is not compatible with our kits, we have developed an AbSelect™ purification kit range that allows you to quickly and simply purify your antibody and is fully compatible with the Lightning-Link® kits.

The appropriate kit to use depends on your particular sample (species, buffer, contaminants, volume, etc.). We have designed a handy flow chart on the AbSelect™ webpage to help you select a kit, but feel free to contact us if you require further guidance. Please consult the individual kit protocols to see the antibody amount/volume suitable for each kit.

If your antibody is already purified but its concentration is significantly lower than 1mg/ml, you can concentrate it using our Antibody Concentration and Clean Up Kit. This kit can also be used to remove low molecular weight contaminants such as azide, tris or glycine by carrying out a buffer exchange into the buffer supplied in the kit, which is fully compatible with Lightning-Link®.

If your antibody contains BSA, you can now use our BSA removal kit to purify your antibody in a few simple steps. Please note this kit will also enable you to concentrate your antibody.

NB: All the AbSelect kits will ONLY work with antibodies. They will not purify other molecules. The only exception is the concentration and clean up kit (861-0010), which will work with other molecules greater than 10kD.

Do I need a wash or desalt step?

One of the advantages of the Lightning-Link® technology compared to traditional labeling methods is that conjugate purification is not required. The Lightning-Link® reactions are highly efficient, and the kits have been optimized so that, provided the protocols are followed, there should be only a very low amount of free label left at the end of the conjugation. This remaining free label would have its reactive 'Lightning-Link®' groups blocked by the Quencher provided in the kit, and is washed away during the wash step of your application.

How stable will my new conjugate be?

Upon dissolution of the Lightning-Link® mixture with a solution of the antibody, proprietary chemicals in the mixture become activated. This results in the directional covalent bonding of the antibody to the label in a gentle and controlled process at near-neutral pH. The stability of your conjugate is dependent on your antibody and label of choice.

Most Lightning-Link® conjugates can be stored for up to 18 months, undiluted at 4°C. The exception to this is tandem conjugates which are only stable for up to 3 months.

Take a look at our absorption-emission table which contains the specifications of all our Lightning-Link® fluorescent dyes. You can view our enzyme and other labels on the Lightning-Link page. You can also view our products categorised by application here.

 

Antibody Purification Kits

Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, and azide) that interfere with labeling reactions. We have developed a range of purification kits which allow for easy and rapid purification of antibodies from any buffer formulation.

AbSelect Antibody Purification Kits

 

Conjugate Check&Go! Kit

Confirm the success of your antibody conjugation with our Conjugate Check&Go! kit, consisting of a nitrocellulose membrane with a 'Test line' of immobilized Protein A and Protein G able to bind to IgG class antibodies from multiple species, a running buffer and a positive control conjugate of immobilized Protein A and Protein G called a “half strip”. The Conjugate Check&Go! strips allow visualization of conjugates generated using our Lightning-Link® fluorescent dyes and proteins.
 

Conjugate Check&Go!

 
Buy Online

3x10ug (703-0030)

In stock

£142.00

1x60ug (703-0005)

In stock

£143.00

3X60ug (703-0010)

In stock

£230.00

1x600ug (703-0015)

In stock

£357.00

5x600ug (703-0003)

In stock

£1,118.00

1x3mg (703-0004)

In stock

£1,055.00
Our shipping times are as follows:
48 hours to the US. 24 to 48 hours to Europe.

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