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ELISA: an introduction to the basic principles and assay formats

The Enzyme-Linked Immunosorbent Assay -ELISA- describes any assay where a molecule is absorbed on a solid phase. This technique allows antibody or analyte detection by their absorption to a solid surface, meaning unbound materials can be washed away with ease.

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This introductory ELISA webinar will discuss the following points:

  1. Introduction to ELISA
  2. The different assay types
  3. Assay development tips
  4. Data analysis tips
  5. Troubleshooting tips
  6. Indirect vs. Direct antibody detection
  7. Using Lightning-Link® for primary antibody labeling
  8. Immuno-PCR

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Did you know Immuno-PCR combines the advantages of ELISA and PCR to offer a powerful method for the detection of small quantities of proteins.? Find out more in our guide ‘Development of an Immuno-PCR assay'!


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