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InnovaCoat® GOLD Nanoparticles & Conjugation Kits

Coated gold nanoparticles and conjugation kits

InnovaCoat® GOLD nanoparticles

InnovaCoat® GOLD nanoparticles have a proprietary surface coating which covalently binds the antibody or protein forming highly stable conjugates. The coated nanoparticles are available within our range of easy-to-use conjugation kits or separately as carboxylated gold nanoparticles.

InnovaCoat® GOLD conjugation kits features and benefits

InnovaCoat® GOLD nanoparticle conjugation kits enable the easy conjugation of gold nanoparticles to antibodies or proteins for use in R&D applications and for the development and manufacture of diagnostic kits.

Features Benefits
Quick and easy to use Save time, no special knowledge required
Different nanoparticle sizes, concentrations and chemistries available Flexible
Freeze dried Ships at ambient temperature, long shelf-life
Fully scalable Easy transfer from R&D to manufacturing
Stringently QC tested Consistent high quality, excellent batch-to-batch reproducibility
Proprietary surface coating Covalent bond, stable conjugates formed
Covalent conjugation uses significantly less antibody than passive conjugation Save precious antibody
Uniform spherical shape and narrow size distribution Batch-to-batch consistency

The kits are designed for optimization purposes, for example, for the screening of potential antibodies or small scale 'proof of principle' studies. Please contact us if you require any additional information or to discuss your bulk or custom conjugation requirements.

Do you want to know if your conjugation was a success?

Try our new and unique Conjugate Check&Go! Kit.

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Standard Vs. Orientated Labeling

The InnovaCoat® GOLD conjugation kits facilitate standard, orientated, or site specific labeling (sites which do not include the binding domain) of IgG antibodies.

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Carboxylated gold nanoparticles

InnovaCoat® GOLD-Carboxyl nanoparticles are coated gold nanoparticles functionalized with carboxyl groups that allow the covalent conjugation of antibodies, using the water soluble carbodiimide crosslinker EDC. Find out more about this product here.

Please do not hesitate to Contact us to find out more!

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    Conjugation Kit data

    Click images to view full screen:


    lateral flow immunoassay, gold nanoparticles


    Figure 1.
    A comparison between the percentage binding of anti-cortisol antibodies conjugated to 40nm gold particles by a traditional passive method against those conjugated covalently using InnovaCoat technology. All components of this competitive lateral flow assay are identical with the exception of the method used to conjugate the antibodies to gold colloid. External data source.

    lateral flow immunoassay, gold nanoparticles

    Figure 2.
    A comparison between the signal intensity, measured on a commercially available lateral flow strip reader, for anti-IgA antibodies conjugated to 40nm gold particles by a traditional passive method against those conjugated covalently using InnovaCoat technology. All components of this competitive lateral flow assay are identical with the exception of the method used to conjugate the antibodies to gold colloid. External data source.

    lateral flow immunoassay, gold nanoparticles

    Figure 3.
    A comparison between the percentage binding of anti-IgA antibodies conjugated to 40nm gold particles by a traditional passive method against those conjugated covalently using InnovaCoat technology. All components of this competitive lateral flow assay are identical with the exception of the method used to conjugate the antibodies to gold colloid. External data source.

    What is different about InnovaCoat® GOLD?

    InnovaCoat® GOLD products are ‘conjugation friendly’ nanoparticles with a proprietary surface coat that greatly enhances gold stability and permits easy covalent attachment of a variety of molecules, including antibodies, analytes and other biomolecules.

    Which different products are available within the InnovaCoat® range?

    The InnovaCoat® range currently consists of the following products:

    Gold nanoparticle conjugation kits: easy-to-use conjugation kits for conjugating antibodies or proteins to coated gold nanoparticles. The conjugation reaction is initiated simply by adding a solution of antibody to the freeze-dried powder; the hands-on time is 2 minutes and the conjugate is ready to use within 15 minutes.

    In the case of immuno-gold conjugates, the antibody can be attached irreversibly without the need for extensive trials at different values of pH and/or salt concentration, as is typical of traditional ‘passive’ binding methods.

    Carboxylated gold nanoparticles: a carboxylated derivative of our InnovaCoat® GOLD 40nm nanoparticles. This product enables the quick generation of highly stable gold-antibody conjugates at R&D and manufacturing scales.

    Conjugated gold nanoparticles: pre-conjugated gold nanoparticles manufactured using our InnovaCoat® GOLD nanoparticles.

    What can I do if my antibody preparation contains interfering substances?

    If your antibody buffer is not compatible with our kits, we have developed the AbPure antibody purification kit range that allows you to quickly and simply purify your antibody and is fully compatible with InnovaCoat® GOLD.

    Please consult the kit protocols to see the antibody amount/volume suitable for each kit.

    Does the antibody bind to the metal surface?

    No. The protective surface coat completely shields the metal surface and prevents direct metal-antibody interactions. For this reason, you cannot use InnovaCoat GOLD for passive conjugation of antibodies.

    Can antibodies from different species be used?

    Yes, the InnovaCoat system has been tested with antibodies from a variety of species including mouse, rabbit, goat and sheep.

    What type of linkage to gold is formed?

    The antibody becomes covalently and irreversibly attached via lysine residues to the InnovaCoat surface.

    Can antibody fragments be conjugated?

    Yes. One of the advantages of the protective coat is that it is less likely than a bare metal surface to cause denaturation and loss of affinity of the antibody fragments.

    What if I need bulk material?

    The InnovaCoat® GOLD conjugation kit is a convenience product for rapid production of small quantities of conjugate for screening purposes. Bulk quantities of InnovaCoat GOLD can be provided in a variety of formats.

    The InnovaCoat® GOLD-Carboxyl nanoparticles enable customers to quickly generate highly stable covalent gold-antibody conjugates at R&D and manufacturing scales.

    Please get in touch for more information on bulk quantities of these products.

    Is the kit suitable for conjugating analytes and other small molecules?

    Yes, but please contact Innova technical services. Depending on the functional groups on your analyte you may need a different type of surface or different chemistry for optimal results.

    Confirm the success of your antibody conjugation with our Check&Go! kit, a nitrocellulose membrane containing a ‘Test line’ of immobilized Protein A and Protein G called a “half strip”. The Conjugate Check&Go! strips allow visualization of conjugates generated using our nanoparticles and beads at different concentrations of antibody.

    Discover more about our ranges of

    gold conjugate 

    InnovaCoat® GOLD Conjugates

    Our pre-conjugated gold nanoparticles are manufactured using our InnovaCoat® GOLD nanoparticles which have a proprietary surface coat that covalently bind the detection protein providing highly stable conjugates. Read More...

     

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    Colloidal gold

    Innova Biosciences colloidal gold nanoparticles are developed using specialized techniques that enable the production of extremely uniform spherical particles with a very narrow size distribution, minimizing variability within your assay. Read More...

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    Purification kits compatible with InnovaCoat® GOLD Nanoparticles & Conjugation Kits

    Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, and azide) that interfere in labeling reactions. We have developed a range of purification kits which allow for easy and rapid purification of antibodies from any buffer formulation. All products are easy to use and are fully compatible with our nanoparticle kits. Read More...

     CheckandGo Kit Image

    Conjugate Check&Go! Kit

    Confirm the success of your antibody conjugation with our Check&Go! kit, a nitrocellulose membrane containing a ‘Test line’ of immobilized Protein A and Protein G called a “half strip”. The Conjugate Check&Go! strips allow visualization of conjugates generated using our beads and nanoparticles at different concentrations of antibody.



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