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Immunogen Preparation Kits

Easy to use immunogen preparation kits for antibody production

immunogen preparation, antibody production


imm-Link immunogen preparation kits for antibody production

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imm-Link kits allow the conjugation of small antigens, often known as haptens, to a carrier protein (such as BSA or Ovalbumin) to maximise an immune response for the generation of antigen reagents.

An antigen is defined as any substance which induces an immune response resulting in the production of antibodies in the host species. The immune response is commonly used to generate antibody (monoclonal and polyclonal) reagents against proteins of interest for use in applications such as Western blotting, immunohistochemistry and flow cytometry.

Haptens tend to be poor at generating an immune response when used alone. In order to maximise a response to such molecules they are often linked to larger "carrier" molecules such as Bovine Serum Albumin (BSA), Ovalbumin or Keyhole Limpet Hemocyanin (KLH), using a covalent chemical bond.

The imm-Link™ conjugation kits allow haptens to be conjugated to a carrier protein simply by adding a solution of the hapten to a lyophilised mixture containing the carrier protein and all the required conjugation chemistry (please see diagram below). The hands-on time to set up the conjugation reaction is typically 20-30 seconds.

immunogen kits

Following successful conjugation the conjugate is dialysed in the supplied dialysis cartridge to remove unwanted by products. The hapten conjugate can then be easily recovered and used.

The imm-Link™ kits are provided in a user friendly format and are capable of targeting different chemistries:

imm-Link™ Immunogen Kit Target Functional Groups
Carboxyl Glutamic acid, aspartic acid or free carboxyl groups
Sulfhydryl Cysteines or free sulfhydryl groups (SH)
Amine Lysines or free amines (NH2)

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