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InnovaCoat® GOLD - 20OD 10nm Gold Conjugation Kits

 

InnovaCoat® GOLD conjugation kits

InnovaCoat® GOLD conjugation kits enable the covalent attachment of antibodies, proteins, peptides and other biomolecules to gold nanoparticles with a proprietary InnovaCoat® coating. The hands-on time is around 30 seconds and the gold conjugate is ready to use after 20 minutes.

InnovaCoat® GOLD conjugation kits features and benefits

Features Benefits
Quick and easy to use Save time, no special knowledge required
Different nanoparticle sizes, concentrations and chemistries available Flexible
Freeze dried Ships at ambient temperature, long shelf-life
Fully scalable Easy transfer from R&D to manufacturing
Stringently QC tested Consistent high quality, excellent batch-to-batch reproducibility
Proprietary surface coating Covalent bond, stable conjugates formed
Covalent conjugation uses significantly less antibody than passive conjugation Save precious antibody
Uniform spherical shape and narrow size distribution Batch-to-batch consistency

Do you want to know if your conjugation was a success?

Try our new and unique Conjugate Check&Go! Kit.

Button-CheckandGo-min


 Please do not hesitate to Contact us to find out more!

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Click images to view full screen:

gold nanoparticles, bioconjugation


Figure 1.
A comparison between the percentage binding of anti-cortisol antibodies conjugated to 40nm gold particles by a traditional passive method against those conjugated covalently using InnovaCoat® technology.  All components of this competitive lateral flow assay are identical with the exception of the method used to conjugate the antibodies to gold colloid.  External data source.

gold nanoparticles, bioconjugation

Figure 2.
A comparison between the signal intensity, measured on a commercially available lateral flow strip reader, for anti-IgA antibodies conjugated to 40nm gold particles by a traditional passive method against those conjugated covalently using InnovaCoat® technology.  All components of this competitive lateral flow assay are identical with the exception of the method used to conjugate the antibodies to gold colloid. External data source.

gold nanoparticles, bioconjugation

Figure 3.
A comparison between the percentage binding of anti-IgA antibodies conjugated to 40nm gold particles by a traditional passive method against those conjugated covalently using InnovaCoat® technology.  All components of this competitive lateral flow assay are identical with the exception of the method used to conjugate the antibodies to gold colloid.  External data source.

 

What can I do if my antibody preparation contains interfering substances?

If your antibody buffer is not compatible with our kits, we have developed the AbPure antibody purification kit range that allows you to quickly and simply purify your antibody and is fully compatible with InnovaCoat® GOLD.

Please consult the kit protocols to see the antibody amount/volume suitable for each kit.

Does the antibody bind to the metal surface?

No. The protective surface coat completely shields the metal surface and prevents direct metal-antibody interactions. For this reason, you cannot use InnovaCoat® GOLD for passive conjugation of antibodies.

Can antibodies from different species be used?

Yes, the InnovaCoat system has been tested with antibodies from a variety of species including mouse, rabbit, goat and sheep.

What type of linkage to gold is formed?

The antibody becomes covalently and irreversibly attached via lysine residues to the InnovaCoat® surface.

Can antibody fragments be conjugated?

Yes. One of the advantages of the protective coat is that it is less likely than a bare metal surface to cause denaturation and loss of affinity of the antibody fragments.

What if I need bulk material?

The kit that you have purchased is a convenience product for rapid production of small quantities of conjugate for screening purposes. Bulk quantities of InnovaCoat GOLD can be provided in a variety of formats. Please enquire.

Is the kit suitable for conjugating analytes and other small molecules?

Yes, but please contact Innova technical services. Depending on the functional groups on your analyte you may need a different type of surface or different chemistry for optimal results.

Confirm the success of your antibody conjugation with our Check&Go! kit, a nitrocellulose membrane containing a ‘Test line’ of immobilized Protein A and Protein G called a “half strip”. The Conjugate Check&Go! strips allow visualization of conjugates generated using our nanoparticles and beads at different concentrations of antibody.

Discover more about our ranges of

innovacoat gold kit components 

InnovaCoat® GOLD Nanoparticles & Conjugation Kits

InnovaCoat® GOLD is a one-step gold nano-particle conjugation kit for antibodies, proteins and peptides or any other biomolecule with an amine group. Read More...

 

 colloidal gold bottle final

Colloidal gold

Innova Biosciences colloidal gold nanoparticles are developed using specialized techniques that enable the production of extremely uniform spherical particles with a very narrow size distribution, minimizing variability within your assay. Read More...

 abpure kit image

Purification kits compatible with InnovaCoat® GOLD Nanoparticles & Conjugation Kits

Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, and azide) that interfere in labeling reactions. We have developed a range of purification kits which allow for easy and rapid purification of antibodies from any buffer formulation. All products are easy to use and are fully compatible with our nanoparticle kits. Read More...

 CheckandGo Kit Image

Conjugate Check&Go! Kit

Confirm the success of your antibody conjugation with our Check&Go! kit, a nitrocellulose membrane containing a ‘Test line’ of immobilized Protein A and Protein G called a “half strip”. The Conjugate Check&Go! strips allow visualization of conjugates generated using our beads and nanoparticles at different concentrations of antibody.
 

Conjugate Check&Go! Kit

Confirm the success of your antibody conjugation with our Check&Go! kit, a nitrocellulose membrane containing a ‘Test line’ of immobilized Protein A and Protein G called a “half strip”. The Conjugate Check&Go! strips allow visualization of conjugates generated using our beads and nanoparticles at different concentrations of antibody.
Product MSDS
Diluent MSDS
Quencher MSDS
Reaction buffer MSDS
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3 Reaction 10nm Mini Kit (228-0005)

In stock

£143.00

10 Reaction 10nm Mini Kit (228-0010)

In stock

£330.00

1 Reaction 10nm Midi Kit (228-0015)

In stock

£330.00
Our shipping times are as follows:
48 hours to the US. 24 to 48 hours to Europe.
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