LATEX Bead Conjugation kits
Latex Bead Conjugation Kits
Our Latex Bead Conjugation Kits are one-step kits for covalently conjugating antibodies, proteins and peptides (or any other biomolecule with an amine group) to specially treated latex beads without the need for extensive optimization.
Our latex conjugation reaction has been developed using our expertise in simple and quick one-step antibody conjugations such as our InnovaCoat® GOLD nanoparticle conjugation kits and our Lightning-Link® antibody labeling kits to produce a product unlike any latex bead conjugation kit available on the market.
Features and Benefits
|Quick and easy to use||Save time, no specialist knowledge required|
|Different colors available||Flexible, allows multiplexing|
|Stringently QC tested||Consistent high quality, excellent batch-to-batch reproducibility|
|Covalent bond||Highly stable conjugates|
|Freeze dried||Ships at ambient temperature, long shelf-life|
|Fully scalable||Easy transfer from R&D to manufacturing|
|No extensive pH optimization required||Save precious antibody / protein|
|Resistant to aggregation||Generation of high quality data|
Do you want to know if your conjugation was a success?
Try our new and unique Conjugate Check&Go! Kit.
Latex conjugation procedure
The researcher simply pipettes the antibody into the vial of lyophilized latex beads and incubates the reaction for 15 minutes. After quenching the reaction, the researcher pellets the latex and removes the buffer. Now the antibody is ready to be resuspended into the storage buffer. The entire procedure takes 3 minutes hands-on time and 35 minutes total time until the conjugates are ready to use.
High yields of functional conjugates can be made without the need for harsh resuspension methods like sonication and vortexing!
Please do not hesitate to Contact us to find out more!
Our Latex conjugation technology is fully scalable for easy transfer from R&D to manufacturing. To request pricing on large quantities please get in touch.
Figure 1. Lateral Flow Assay of our specially-treated latex beads conjugated to a monoclonal anti-CRP antibody (mAb1) titrated against CRP. 2 ng/ml CRP can easily be detected.
Figure 2. Lateral Flow Assay of our specially-treated latex conjugated to the monoclonal anti-CRP antibody (mAb 2) titrated against CRP in buffer or CRP spiked CRP-depleted serum (100 % serum). The latex behaves similarly in serum and buffer, and no aggregation or non-specific binding is seen.
Figure 3. Multiplexed latex. The three colors of latex each conjugated to a different antibody or protein and forming a line either by a direct binding event (red and black latex) or a sandwich assay binding event (blue latex). The three colors of latex demonstrate no aggregation or background staining.
Our Black Latex Conjugation Kits are available both as Mini and Midi kits. Mini kits are ideal for antibody screening or ‘proof of principle’ experiments, while the Midi kits are 10 times the size of our Mini kits. Bulk material is also available for further scale up.
Our Blue Latex Conjugation Kits are available both as Mini and Midi kits. Mini kits are ideal for antibody screening or ‘proof of principle’ experiments, while the Midi kits are 10 times the size of our Mini kits. Bulk material is also available for further scale up.
Our Red Latex Conjugation Kits are available both as Mini and Midi kits. Mini kits are ideal for antibody screening or ‘proof of principle’ experiments, while the Midi kits are 10 times the size of our Mini kits. Bulk material is also available for further scale up.
Is your antibody too dilluted or not in a suitable buffer? Consider using our Antibody Clean Up kit specifically designed for Latex applications.
Confirm the success of your antibody conjugation with our Check&Go! kit, a nitrocellulose membrane containing a ‘Test line’ of immobilized Protein A and Protein G called a “half strip”. The Conjugate Check&Go! strips allow visualization of conjugates generated using our beads and nanoparticles at different concentrations of antibody.
The latex particles are specially treated to enhance the handling of the latex and permit easy covalent attachment of antibodies and other proteins. The conjugation reaction is initiated simply by adding a solution of the antibody to the freeze-dried powder; the hands on time is 3 minutes and the conjugate is ready to use within 35 minutes.
Latex nanoparticles are prone to aggregation both before and after conjugation, and harsh methods such as sonication or vortexing are routinely needed to resuspend both conjugated and unconjugated latex. Our special treatment significantly improves the handling of the latex and vastly reduces the propensity of the latex to aggregate. Harsh resuspension methods are not required, and recovery of usual mono-disperse latex particles is very high.
TThe antibody becomes covalently attached to the specially treated surface via lysine residues.
The special treatment shields the latex surface, preventing surface - antibody interactions. For this reason you cannot use our latex for passive conjugation.
Each vial of freeze dried latex will provide 40ul 1% conjugated latex. In the 4 reaction kit there is 160ul 1% latex in total and in the 10 reaction kit there is a total of 400ul 1% latex. 40ul 1% latex should provide enough conjugate to perform approximately 50 lateral flow assays.
The Mini kits are a convenient for rapid production of small quantities of conjugate for screening purposes. The Midi kits are ten times the size of the Mini kits for medium scale work (e.g. ~500 lateral flow tests). Bulk quantities can also be produced to meet your custom needs. Please contact us to discuss your bulk or custom conjugation needs or if you require any additional information.
Yes. We have tested a variety of species including mouse & goat.
Yes. Our Latex Conjugation Kit has been developed for use with both monoclonal and polyclonal antibodies.
One of the advantages of the protective coat is that it is less likely than a bare surface to cause denaturation of antibody fragments and other proteins.
The Latex Conjugation Kit has been optimised for use with antibodies, but it may be possible to conjugate small molecules with primary amine functional groups. Please contact Innova Technical Services at firstname.lastname@example.org for information and support.
The conjugation reaction is sensitive to a number of common buffers and buffer additives. The antibody should be in 10 – 50 mM MES, MOPS or HEPES at pH 6 – 7 for conjugation. As these are not common storage buffers for antibodies and other proteins we have developed the companion product the Antibody Concentration & Clean Up Kit for Latex to switch the antibody buffer to one that is optimal for conjugation efficiency.
To remove contaminating proteins before conjugation or to purify your antibody from ascites fluid, serum or tissue culture supernatant please see our AbPure range of purification products.
Confirm the success of your antibody conjugation with our Check&Go! kit, a nitrocellulose membrane containing a ‘Test line’ of immobilized Protein A and Protein G called a “half strip”. The Conjugate Check&Go! strips allow visualization of conjugates generated using our latex beads at different concentrations of antibody.
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