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LATEX Bead Conjugation kits

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Latex Bead Conjugation Kits

Our Latex Bead Conjugation Kits are one-step kits for covalently conjugating antibodies, proteins and peptides (or any other biomolecule with an amine group) to specially treated latex beads without the need for extensive optimization.

Our latex conjugation reaction has been developed using our expertise in simple and quick one-step antibody conjugations such as our InnovaCoat® GOLD nanoparticle conjugation kits and our Lightning-Link® antibody labeling kits to produce a product unlike any latex bead conjugation kit available on the market.

Features and Benefits

Features Benefits
Quick and easy to use Save time, no specialist knowledge required
Different colors available Flexible, allows multiplexing
Stringently QC tested Consistent high quality, excellent batch-to-batch reproducibility
Covalent bond Highly stable conjugates
Freeze dried Ships at ambient temperature, long shelf-life
Fully scalable Easy transfer from R&D to manufacturing
No extensive pH optimization required Save precious antibody / protein
Resistant to aggregation Generation of high quality data

Do you want to know if your conjugation was a success?

Try our new and unique Conjugate Check&Go! Kit.

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Latex conjugation procedure

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The researcher simply pipettes the antibody into the vial of lyophilized latex beads and incubates the reaction for 15 minutes. After quenching the reaction, the researcher pellets the latex and removes the buffer. Now the antibody is ready to be resuspended into the storage buffer. The entire procedure takes 3 minutes hands-on time and 35 minutes total time until the conjugates are ready to use. 

High yields of functional conjugates can be made without the need for harsh resuspension methods like sonication and vortexing!

Please do not hesitate to Contact us to find out more!

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Bulk Quantities

Our Latex conjugation technology is fully scalable for easy transfer from R&D to manufacturing. To request pricing on large quantities please get in touch.

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Data

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Figure 1. Lateral Flow Assay of our specially-treated latex beads conjugated to a monoclonal anti-CRP antibody (mAb1) titrated against CRP. 2 ng/ml CRP can easily be detected.

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Figure 2. Lateral Flow Assay of our specially-treated latex conjugated to the monoclonal anti-CRP antibody (mAb 2) titrated against CRP in buffer or CRP spiked CRP-depleted serum (100 % serum). The latex behaves similarly in serum and buffer, and no aggregation or non-specific binding is seen.

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Figure 3.  Multiplexed latex. The three colors of latex each conjugated to a different antibody or protein and forming a line either by a direct binding event (red and black latex) or a sandwich assay binding event (blue latex). The three colors of latex demonstrate no aggregation or background staining.

Related products

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Black Latex Conjugation Kit

Our Black Latex Conjugation Kits are available both as Mini and Midi kits. Mini kits are ideal for antibody screening or ‘proof of principle’ experiments, while the Midi kits are 10 times the size of our Mini kits. Bulk material is also available for further scale up.

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Blue Latex Conjugation Kit

Our Blue Latex Conjugation Kits are available both as Mini and Midi kits. Mini kits are ideal for antibody screening or ‘proof of principle’ experiments, while the Midi kits are 10 times the size of our Mini kits. Bulk material is also available for further scale up.

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Red Latex Conjugation Kit

Our Red Latex Conjugation Kits are available both as Mini and Midi kits. Mini kits are ideal for antibody screening or ‘proof of principle’ experiments, while the Midi kits are 10 times the size of our Mini kits. Bulk material is also available for further scale up.

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4 reaction Antibody Clean Up Kit for Latex

Is your antibody too dilluted or not in a suitable buffer? Consider using our Antibody Clean Up kit specifically designed for Latex applications.

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Conjugate Check&Go! Kit

Confirm the success of your antibody conjugation with our Check&Go! kit, a nitrocellulose membrane containing a ‘Test line’ of immobilized Protein A and Protein G called a “half strip”. The Conjugate Check&Go! strips allow visualization of conjugates generated using our beads and nanoparticles at different concentrations of antibody.

What is different about the Latex Conjugation Kit?

The latex particles are specially treated to enhance the handling of the latex and permit easy covalent attachment of antibodies and other proteins. The conjugation reaction is initiated simply by adding a solution of the antibody to the freeze-dried powder; the hands on time is 3 minutes and the conjugate is ready to use within 35 minutes.

Latex nanoparticles are prone to aggregation both before and after conjugation, and harsh methods such as sonication or vortexing are routinely needed to resuspend both conjugated and unconjugated latex. Our special treatment significantly improves the handling of the latex and vastly reduces the propensity of the latex to aggregate. Harsh resuspension methods are not required, and recovery of usual mono-disperse latex particles is very high.

What type of linkage to the latex is formed?

TThe antibody becomes covalently attached to the specially treated surface via lysine residues.

Does the conjugated antibody bind to the latex surface?

The special treatment shields the latex surface, preventing surface - antibody interactions. For this reason you cannot use our latex for passive conjugation.

How many latex beads are in each kit?

Each vial of freeze dried latex will provide 40ul 1% conjugated latex. In the 4 reaction kit there is 160ul 1% latex in total and in the 10 reaction kit there is a total of 400ul 1% latex. 40ul 1% latex should provide enough conjugate to perform approximately 50 lateral flow assays.

What if I need bulk material?

The Mini kits are a convenient for rapid production of small quantities of conjugate for screening purposes. The Midi kits are ten times the size of the Mini kits for medium scale work (e.g. ~500 lateral flow tests).  Bulk quantities can also be produced to meet your custom needs. Please contact us to discuss your bulk or custom conjugation needs or if you require any additional information.

Can antibodies from different species be used?

Yes. We have tested a variety of species including mouse & goat.

Can monoclonal and polyclonal antibodies be used?

Yes. Our Latex Conjugation Kit has been developed for use with both monoclonal and polyclonal antibodies.

Can antibody fragments and non-antibody proteins be conjugated?

One of the advantages of the protective coat is that it is less likely than a bare surface to cause denaturation of antibody fragments and other proteins.

Is the kit suitable for conjugating small molecules including analytes?

The Latex Conjugation Kit has been optimised for use with antibodies, but it may be possible to conjugate small molecules with primary amine functional groups. Please contact Innova Technical Services at tech@innovabiosciences.com for information and support.

What can I do if my antibody is not in a compatible buffer?

The conjugation reaction is sensitive to a number of common buffers and buffer additives. The antibody should be in 10 – 50 mM MES, MOPS or HEPES at pH 6 – 7 for conjugation. As these are not common storage buffers for antibodies and other proteins we have developed the companion product the Antibody Concentration & Clean Up Kit for Latex to switch the antibody buffer to one that is optimal for conjugation efficiency.

What can I do if my antibody has non-antibody protein components or is not purified?

To remove contaminating proteins before conjugation or to purify your antibody from ascites fluid, serum or tissue culture supernatant please see our AbPure range of purification products.

What colour latex should I buy?

Our blue, red and black latex all behave in a very similar way chemically, so the ideal latex colour will depend on your application and the preference of the scientist.

Confirm the success of your antibody conjugation with our Check&Go! kit, a nitrocellulose membrane containing a ‘Test line’ of immobilized Protein A and Protein G called a “half strip”. The Conjugate Check&Go! strips allow visualization of conjugates generated using our latex beads at different concentrations of antibody.







Conjugate Check&Go! Kit

Confirm the success of your antibody conjugation to fluorescent dyes and proteins, latex beads and gold nanoparticles. This kit is compatible with our Lightning-Link®, InnovaCoat® GOLD and Latex Bead conjugation technologies as well as other labeling technologies available commercially.


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