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Phosphate Detection

Methods for measuring inorganic phosphate (Pi) using dyes such as Malachite Green are well known, however the dye-Pi complexes are prone to precipitation and the reagents are very acidic which can lead to non-enzymatic hydrolysis of many phosphorylated substrates.

At the heart of the Innova Biosciences' range of phosphate detection products is PiColorLockTM a unique phosphate detection reagent that overcomes these common issues. The formulation of PiColorLockTM affords enhanced assay linearity, dynamic range and color stability, and it is also possible to work with unstable substrates that give high non-enzymatic background signals with other acidic dye-based detection reagents.

PiColorLockTM can be purchased singularly or as part of our ATPase and GTPase assay kits.

For more information about our range of phosphate detection products, take a look at our video below:

 



High Throughput Colorimetric ATPase Assays (inc. PiColorLock)

This non radioactive colorimetric assay kit contains all the necessary reagents for measuring enzyme activity (everything included in the PiColorlock kit and more) and is ideal for high throughput drug screening.

10mM Lyophilized GTP

These lyophilized 10mM GTP vials contain ultra high quality GTP to ensure the lowest possible assay background. (Also supplied as part of the GTPase assay kit).

PiBind™ resin

PiBind™ resin provides a quick and easy way to remove contaminating inorganic phosphate (Pi) from buffers.

High Throughput Colorimetric GTPase Assays (inc. PiColorLock)

This non radioactive colorimetric assay kit contains all the necessary reagents for measuring enzyme activity (everything included in the PiColorlock kit and more) and is ideal for high throughput drug screening.

PiColorLock™ Phosphate Detection System

PiColorLock is a phosphate detection reagent for measuring phosphatases, ATPases, GTPases and other enzymes that release inorganic phosphate (Pi). The PiColorLock Gold Phosphate Detection System includes PiColorLock Gold reagent (high sensitivity) as well as Accelerator, Stabiliser and Pi Standard.

10mM Lyophilized ATP

These lyophilized 10mM ATP vials contain ultra high quality ATP to ensure the lowest possible assay background. (Also supplied as part of the ATPase assay kit).


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