Protein A – Alkaline Phosphatase
Protein A is a 42 kDa protein originally found in S. aureus, constituted by five homologous domains which bind the Fc region of many mammalian IgGs. Its high affinity to immunoglobulins is the reason why it is largely used in biochemical research.
Alkaline Phosphatase is extracted from bovine intestine and purified using multiple precipitation, fractionation, chromatography, and filtration steps. The hydrolyase enzyme is frequently used in immunoassays. PNPP is a popular colorimetric substrate for it; the product of the reaction, p-nitrophenol, can be read at 405nm. Fluorogenic substrates (e.g. 4- methylumbelliferyl phosphate; MUP) may also be employed.
All of our conjugates are produced using our Lightning-Link® bioconjugation technology, therefore providing high quality, stable conjugates for use in your application.
For added flexibility we provide the conjugates in two different pack sizes of 1mg and 100ug. Furthermore the conjugates are freeze-dried so shipping at ambient temperature is not a problem.
For more information or to ask a technical questions please get in touch at firstname.lastname@example.org.
|Specificity||Most mammalian IgGs|
|Host species||E.coli and Bovine|
|Product size||100ug and 1mg total protein|