Protein A – Alkaline Phosphatase
Protein A - Alkaline Phosphatase conjugate
Innova Biosciences' Protein A - Alkaline Phosphatase conjugates are manufactured using our Lightning-Link® antibody labeling technology, therefore providing extremely high quality, stable conjugates.
For added flexibility we provide the conjugates in two different pack sizes of 100ug and 1mg. Furthermore the conjugates are freeze-dried so shipping at ambient temperature is not a problem.
|Specificity||Most mammalian IgGs|
|Host species||E.coli and Bovine|
|Product size||100ug and 1mg total protein|
Fluorescence intensity of Innova Biosciences' Protein A - Alkaline Phosphatase conjugate versus competitor
Figure 1. A Protein A - Alkaline Phosphatase conjugate was purchased from a commercial supplier. The conjugate was compared to Protein A - Alkaline Phosphatase from Innova Biosciences in an ELISA. It can be seen that the Innova Biosciences' Protein A - Alkaline Phosphatase conjugate outperforms the competitor.
Protein A is a 42kDa surface protein of the bacterium Staphylococcus aureus, which binds the Fc region of many mammalian IgGs. Its high affinity for immunoglobulins is the reason that it is often used in biochemical research.
Alkaline Phosphatase is a hydrolase enzyme. It is extracted from bovine intestine and purified using multiple precipitation, fractionation, chromatography, and filtration steps. Alkaline Phosphatase is frequently employed in immunoassays, and a commonly used colorimetric substrate for it is PNPP; the product of this reaction, p-nitrophenol, can be read at 405nm. Fluorogenic substrates such as 4-methylumbelliferyl phosphate (MUP) may also be used.