Protein A - HRP
Protein A - Horseradish Peroxidase conjugate
Innova Biosciences' Protein A - Horseradish Peroxidase conjugates are manufactured using our Lightning-Link® antibody labeling technology, therefore providing extremely high quality, stable conjugates. For added flexibility we provide the conjugates in two different pack sizes of 100ug and 1mg. Furthermore the conjugates are freeze-dried so shipping at ambient temperature is not a problem.
|Specificity||Most mammalian IgGs|
|Host species||E.coli and Horseradish|
|Product size||100ug and 1mg total protein|
Fluorescence intensity of Innova Biosciences' Protein A - Horseradish Peroxidase conjugate versus competitor
Figure 1. A Protein A - Horseradish Peroxidase conjugate was purchased from a commercial supplier. The conjugate was compared to Protein A - Horseradish Peroxidase from Innova Biosciences in an ELISA. It can be seen that the Innova Biosciences protein A - Horseradish Peroxidase conjugate outperforms the competitor.
Protein A is a 42kDa surface protein of the bacterium Staphylococcus aureus, which binds the Fc region of many mammalian IgGs. Its high affinity for immunoglobulins is the reason that it is often used in biochemical research.
Horseradish Peroxidase (HRP) is a metalloenzyme which has many different isoforms. It utilises hydrogen peroxide to oxidize both organic and inorganic compounds, and in the presence of the appropriate substrate (e.g. TMB, DAB, ABTS) causes a color change that can be measured spectrophotometrically.