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Streptavidin – Alkaline Phosphatase

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Streptavidin-Alkaline Phosphatase (AP) enzyme conjugate

Innova Biosciences' Streptavidin-Alkaline Phosphatase conjugates are manufactured using our Lightning-Link® antibody labeling technology, therefore providing extremely high quality, stable conjugates.

For added flexibility we provide the conjugates in two different pack sizes of 100ug and 1mg. Furthermore the conjugates are freeze-dried so shipping at ambient temperature is not a problem.

Specifications

Specificity Biotin
Host species E.coli and Bovine
Format Lyophilized
Product size  100ug and 1mg total protein
Buffer Phosphate Buffer
Storage -20°C

Comparative data

Streptavidin-AP vs competitors

Figure 1. A Streptavidin-Alkaline Phosphatase conjugate was purchased from a commercial supplier. The conjugate was compared to Streptavidin-Alkaline Phosphatase conjugate made with Innova Biosciences' Lightning-Link® conjugation technology in ELISA as shown. It can be seen that the Innova Biosciences' Streptavidin-Alkaline Phosphatase conjugate outperforms the competitor.

Related products

Streptavidin – R Phycoerythrin (R-PE)   [find out more]

Streptavidin – Horseradish Peroxidase (HRP)   [find out more]

Streptavidin – Fluorescein   [find out more]

Lightning-Link® Rapid Biotin  [find out more]

Directly conjugate your antibody, protein or any other biomolecule with an available amine group in under 20 minutes with only 30 seconds hands-on time. Label from 10ug to a gram or more and recover 100% of your antibody.

Additional information

Streptavidin is purified from the bacterium Streptomyces avidinii. It is a tetrameric protein that is composed of identical subunits, with the tetramer having a molecular weight of approximately 53kDa. Each subunit binds one biotin molecule with a very high degree of specificity and affinity. The Kd for this interaction is in the region of 10-15M.

Alkaline Phosphatase is a hydrolase enzyme. It is extracted from bovine intestine and purified using multiple precipitation, fractionation, chromatography, and filtration steps. Alkaline Phosphatase is frequently employed in immunoassays, and a commonly used colorimetric substrate for it is PNPP; the product of this reaction, p-nitrophenol, can be read at 405nm. Fluorogenic substrates such as 4-methylumbelliferyl phosphate (MUP) may also be used.

Buy Online

100ug (2011-0100)

In stock

£94.00

1mg (2011-1000)

In stock

£188.00
Our shipping times are as follows:
48 hours to the US. 24 to 48 hours to Europe.

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