Streptavidin – Alkaline Phosphatase
Streptavidin-Alkaline Phosphatase (AP) enzyme conjugate
Innova Biosciences' Streptavidin-Alkaline Phosphatase conjugates are manufactured using our Lightning-Link® antibody labeling technology, therefore providing extremely high quality, stable conjugates.
For added flexibility we provide the conjugates in two different pack sizes of 100ug and 1mg. Furthermore the conjugates are freeze-dried so shipping at ambient temperature is not a problem.
|Host species||E.coli and Bovine|
|Product size||100ug and 1mg total protein|
Figure 1. A Streptavidin-Alkaline Phosphatase conjugate was purchased from a commercial supplier. The conjugate was compared to Streptavidin-Alkaline Phosphatase conjugate made with Innova Biosciences' Lightning-Link® conjugation technology in ELISA as shown. It can be seen that the Innova Biosciences' Streptavidin-Alkaline Phosphatase conjugate outperforms the competitor.
Streptavidin is purified from the bacterium Streptomyces avidinii. It is a tetrameric protein that is composed of identical subunits, with the tetramer having a molecular weight of approximately 53kDa. Each subunit binds one biotin molecule with a very high degree of specificity and affinity. The Kd for this interaction is in the region of 10-15M.
Alkaline Phosphatase is a hydrolase enzyme. It is extracted from bovine intestine and purified using multiple precipitation, fractionation, chromatography, and filtration steps. Alkaline Phosphatase is frequently employed in immunoassays, and a commonly used colorimetric substrate for it is PNPP; the product of this reaction, p-nitrophenol, can be read at 405nm. Fluorogenic substrates such as 4-methylumbelliferyl phosphate (MUP) may also be used.