Streptavidin – Alkaline Phosphatase
Streptavidin has a molecular weight of 53kDa and is purified from Streptomyces avidinii. It is a tetrameric protein composed of identical subunits. Each subunit binds one biotin molecule with a high degree of affinity and specificity with KD of ~1x10-15M which makes it one of the strongest known non-covalent interactions. Due to this high affinity it has widespread applications.
Alkaline Phosphatase is extracted from bovine intestine and purified using multiple precipitation, fractionation, chromatography, and filtration steps. The hydrolyase enzyme is frequently used in immunoassays. PNPP is a popular colorimetric substrate for Alkaline Phosphatase; the product of the reaction, p-nitrophenol, can be read at 405nm. Fluorogenic substrates (e.g. 4- methylumbelliferyl phosphate; MUP) may also be employed.
All of our conjugates are produced using our Lightning-Link® bioconjugation technology, therefore providing high quality, stable conjugates for use in your application.
For added flexibility we provide the conjugates in two different pack sizes of 1mg and 100ug. Furthermore the conjugates are freeze-dried so shipping at ambient temperature is not a problem.
For more information or to ask a technical questions please get in touch at firstname.lastname@example.org.
|Host species||S. avidinii and Bovine|
|Product size||100ug and 1mg total protein|