Streptavidin – HRP
Streptavidin has a molecular weight of 53kDa and is purified from Streptomyces avidinii. It is a tetrameric protein composed of identical subunits. Each streptavidin monomer binds one biotin molecule with a very high affinity (dissociation constant (Kd) ≈10−14 mol/L) and specificity. It has been demonstrated that the Streptavidin – Biotin interaction is more specific than Avidin – Biotin.
Streptavidin-HRP is used in conjunction with biotinylated primary antibodies. Their interaction can be detected by the Horseradish Peroxidase (HRP). HRP is a metalloenzyme that in presence of the appropriate substrate (e.g., TMB, DAB, ABTS) causes its oxidation. The colour switch due to such chemical reaction can be easily measured by spectrophotometric methods.
All of our conjugates are produced using our Lightning-Link® bioconjugation technology, therefore providing high quality, stable conjugates for use in your application.
For added flexibility we provide the conjugates in two different pack sizes of 1mg and 100ug. Furthermore the conjugates are freeze-dried so shipping at ambient temperature is not a problem.
For more information or to ask a technical questions please get in touch at firstname.lastname@example.org.
|Host species||E.coli and Horseradish|
|Product size||100ug and 1mg Streptavidin|