Streptavidin – R-Phycoerythrin
Streptavidin - R-PE fluorescent conjugate
Innova Biosciences' Streptavidin - R-Phycoerythrin conjugates are manufactured using our Lightning-Link® antibody labeling technology, therefore providing extremely high quality, stable conjugates.
For added flexibility we provide the conjugates in two different pack sizes of 100ug and 1mg. Furthermore the conjugates are freeze-dried so shipping at ambient temperature is not a problem.
|Host species||E.coli and Red Algae|
|Product size||100ug and 1mg total protein|
Fluorescence intensity of Innova Biosciences' Streptavidin - R-Phycoerythrin conjugate versus competitor
Figure 1. A Streptavidin - R-Phycoerythrin conjugate was purchased from a commercial supplier. The conjugate was compared to Streptavidin - R-PE conjugate made with Innova Biosciences' Lightning-Link® conjugation technology in ELISA as shown. It can be seen that the Innova Biosciences' Streptavidin - R-PE conjugate outperforms the competitor.
Streptavidin is purified from the bacterium Streptomyces avidinii. It is a tetrameric protein that is composed of identical subunits, with the tetramer having a molecular weight of approximately 53kDa. Each subunit binds one biotin molecule with a very high degree of specificity and affinity. The Kd for this interaction is in the region of 10-15M.
R-Phycoerythrin (R-PE) is a 240kDa phycobiliprotein that is isolated from red algae. R-PE has a bright red-orange fluorescence with a high quantum yield, and is brighter than other conventional fluophores and phycobiliproteins. The absorbance spectrum of R-PE covers a range of excitation wavelengths, which provides an advantage for multi-color immunofluorescent staining or cell-sorting. The emission maximum of R-PE is at 575nm. R-PE is used for fluorescence-based detection, often in assays which require high sensitivity.