Streptavidin – R-Phycoerythrin
Streptavidin has a molecular weight of 53kDa and is purified from Streptomyces avidinii. It is a tetrameric protein composed of identical subunits. Each streptavidin monomer binds one biotin molecule with a very high affinity (dissociation constant (Kd) ≈10−14 mol/L) and specificity. The strength and specificity of the interaction has led it to be one of the most widely used affinity pairs in molecular, immunological, and cellular assays. This interaction can be detected by the fluorescent molecule.
R-PE is a 240kDa fluorescent protein from the phycobiliprotein family that is isolated from red algae. R-PE has a bright orange fluorescence with a high quantum yield, giving a brighter fluorescence than other conventional fluorophores and phycobiliproteins. The absorbance spectrum of R-PE covers a broad range of excitation wavelengths, and its emission maximum is at 575nm. The broad range of excitation wavelengths provides an advantage for multi-colour immunofluorescent staining or cell sorting.
All of our conjugates are produced using our Lightning-Link® bioconjugation technology, therefore providing high quality, stable conjugates for use in your application.
For added flexibility we provide the conjugates in two different pack sizes of 1mg and 100ug. Furthermore the conjugates are freeze-dried so shipping at ambient temperature is not a problem.
For more information or to ask a technical questions please get in touch at firstname.lastname@example.org.
|Host species||E.coli and Red Algae|
|Product size||100ug and 1mg total protein|