InnovaCoat® GOLD - 40nm Biotin gold conjugate (10OD)
InnovaCoat® GOLD is a unique coated nanoparticle that has an avidly bound protective surface coat which can withstand the most extreme conditions (e.g. 2.5M NaOH at 70oC for ~2 hours).
By covalently attaching biotin ligand to the surface coat the resulting InnovaCoat® GOLD-Biotin nanoparticles inherit the stability attributes of the core nanoparticle; moreover the biotin ligand cannot desorb, which is a major problem with all competing products in which a biotin analogue is directly attached to the metal.
OD (concentration): 10
Size of gold: 40 nm
InnovaCoat® GOLD Biotin demonstrates enhanced sensitivity compared to competitor products in lateral flow assays.
The graph shows a comparison of test and control line signal intensities between conjugated gold nanoparticles from Competitor 1 or 2 and covalently conjugated InnovaCoat® GOLD-Biotin on lateral flow test strips.
The amount of streptavidin labeled antibody used in InnovaCoat® GOLD biotin in lateral flow assays should be optimised. In the following experiment a streptavidin conjugated rabbit IgG antibody was used, in combination with InnovaCoat® GOLD biotin, in a lateral flow assay with a test (t) line (Goat anti Rabbit IgG- GAR) and a control (c) line (biotin).
- Scenario 1 – Streptavidin conjugated Rabbit IgG (5-50 ?g/ml) binds to the Biotin GOLD producing a strong signal on the Test Line.
- Scenario 2 – Excess Streptavidin conjugated Rabbit IgG (400 ?g/ml) results in competition between biotin Gold bound antibody and free antibody for the test line, leading to a weaker signal. The excess leads to binding of Biotin Gold linked to streptavidin conjugated rabbit IgG to the control line.
- Scenario 3 – a lack of Streptavidin conjugated Rabbit IgG (1-10 ?g/ml) leads to a very weak test line.
Diagrammatic representation of the three scenarios
Experimental data demonstrating the three scenarios