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InnovaCoat® GOLD - 40nm Biotin gold conjugate (10OD)

InnovaCoat® GOLD is a unique coated nanoparticle that has an avidly bound protective surface coat which can withstand the most extreme conditions (e.g. 2.5M NaOH at 70oC for ~2 hours).

By covalently attaching biotin ligand to the surface coat the resulting InnovaCoat® GOLD-Biotin nanoparticles inherit the stability attributes of the core nanoparticle; moreover the biotin ligand cannot desorb, which is a major problem with all competing products in which a biotin analogue is directly attached to the metal.

Biotin is attached to InnovaCoat® GOLD via an extended linker to facilitate high affinity molecular interactions with avidin and streptavidin.

OD (concentration): 10
Size of gold: 40 nm

InnovaCoat® GOLD Biotin demonstrates enhanced sensitivity compared to competitor products in lateral flow assays.

innovacoat biotin 300 web

Figure 4.
The graph shows a comparison of test and control line signal intensities between conjugated gold nanoparticles from Competitor 1 or 2 and covalently conjugated InnovaCoat® GOLD-Biotin on lateral flow test strips.

The graph shows a comparison of test and control line signal intensities between conjugated gold nanoparticles from Competitor 1 or 2 and covalently conjugated InnovaCoat® GOLD-Biotin on lateral flow test strips.

Technical Tip

The amount of streptavidin labeled antibody used in InnovaCoat® GOLD biotin in lateral flow assays should be optimised. In the following experiment a streptavidin conjugated rabbit IgG antibody was used, in combination with InnovaCoat® GOLD biotin, in a lateral flow assay with a test (t) line (Goat anti Rabbit IgG- GAR) and a control (c) line (biotin).

  • Scenario 1 – Streptavidin conjugated Rabbit IgG (5-50 ?g/ml) binds to the Biotin GOLD producing a strong signal on the Test Line.
  • Scenario 2 – Excess Streptavidin conjugated Rabbit IgG (400 ?g/ml) results in competition between biotin Gold bound antibody and free antibody for the test line, leading to a weaker signal. The excess leads to binding of Biotin Gold linked to streptavidin conjugated rabbit IgG to the control line.
  • Scenario 3 – a lack of Streptavidin conjugated Rabbit IgG (1-10 ?g/ml) leads to a very weak test line.

Diagrammatic representation of the three scenarios

 lateral flow

Experimental data demonstrating the three scenarios

lateral flow immunoassay

If you haven't found what you're looking for, click here for our gold nanoparticle and gold conjugation kits.

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0.2ml (240-0200)

In stock

£176.00

1ml (240-1000)

In stock

£474.00
Our shipping times are as follows:
48 hours to the US. 24 to 48 hours to Europe.
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