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InnovaCoat® GOLD - 40nm Streptavidin gold conjugate (10OD)

InnovaCoat® GOLD is a unique coated nanoparticle that has an avidly bound protective surface coat which can withstand the most extreme conditions (e.g. 2.5M NaOH at 70oC for ~2 hours).

By covalently attaching streptavidin to the surface coat the resulting InnovaCoat® GOLD-Streptavidin nanoparticles inherit the stability attributes of the core nanoparticle; moreover the streptavidin ligand cannot desorb, which is a major problem with all the competing products in which the streptavidin is directly attached to the metal.

OD (concentration): 10
Size of gold: 40 nm

Technical Tip

The amount of biotin labeled antibody used in InnovaCoat® GOLD streptavidin in lateral flow assays should be optimised. In the following experiment a biotin conjugated rabbit IgG antibody was used, in combination with InnovaCoat® GOLD streptavidin, in a lateral flow assay with a test (t) line (Goat anti Rabbit IgG- GAR) and a control (c) line (biotin).

  • Scenario 1 – Biotin conjugated Rabbit IgG (50-100 ?g/ml) binds to the streptavidin GOLD producing a strong signal on the Test Line.
  • Scenario 2 – Excess biotin conjugated Rabbit IgG (>400 ?g/ml) results in competition between streptavidin Gold bound antibody and free antibody for the test line, leading to a weaker signal. The excess leads to binding of streptavidin Gold linked to biotin conjugated rabbit IgG to the control line.
  • Scenario 3 – A lack of biotin conjugated Rabbit IgG leads to a very weak test line.

Diagrammatic representation of the three scenarios

 lateral flow assay

Experimental data demonstrating the three scenarios

lateral flow

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0.2ml (250-0200)

In stock

£184.00

1ml (250-1000)

In stock

£485.00
Our shipping times are as follows:
48 hours to the US. 24 to 48 hours to Europe.
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